Virus Concentration Using Sulfonated Magnetic Beads to Improve Sensitivity in Nucleic Acid Amplification Tests(Analytical Biochemistry)

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概要

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• To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration method using sulfonated (SO-) magnetic beads in the presence of divalent cations. In the presence of either Zn^<2+> or Cu^<2+> ions, we showed that SO-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine parvovirus (PPV) and poliovirus, which were not concentrated by polyethyleneimine (PEI)-magnetic beads.^<1)> Using the SO-magnetic beads, the sensitivity of virus genome detection by PCR or RT-PCR can be enhanced. Therefore, an efficient virus concentration method using either SO-magnetic beads or PEI-magnetic beads enhances the sensitivity of virus detection by PCR or RT-PCR.

• 公益社団法人日本薬学会の論文
• 2003-08-01

著者

• Yamaguchi Teruhide

  The National Institute Of Health Sciences

• Hayakawa Takao

  The National Institute Of Health Sciences

• Iwata A

  The National Institute Of Health Sciences

• IWATA Akiko

  The National Institute of Health Sciences

• SATOH Koei

  The Institute of Saitama Red Cross Center

• MURATA Mitsuhiro

  JSR Corporation, Tsukuba Research Laboratories

• HIKATA Mikio

  JSR Corporation, Tsukuba Research Laboratories

• Hikata Mikio

  Jsr Corporation Tsukuba Research Laboratories

• Murata Mitsuhiro

  Jsr Corporation Tsukuba Research Laboratories

関連論文

• Virus Concentration Using Sulfonated Magnetic Beads to Improve Sensitivity in Nucleic Acid Amplification Tests(Analytical Biochemistry)

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