Applications of MTT Assay to Primary Cultured Rat Hepatocytes
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概要
- 論文の詳細を見る
We investigated the optimal conditions for the application of the MTT assay to determine the viability of cell in primary cultures of rat hepatocytes. The optimal conditions were found to be : inoculation at cell density of 1.31×10^5 cells/cm^2 ; concentration of 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 0.12mM ; and MTT incorporation time, 2 h or more. We also found that insulin and glucocorticoid, which are usually added to the medium to maintain cell function, had no effect on the viability of the primary cultures of rat hepatocytes.
- 公益社団法人日本薬学会の論文
- 1995-10-15
著者
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堀 均
Biological Science And Technology Faculty Of Engineering University Of Tokushima
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横山 英輝
Tag Biosystem Institute:biological Science And Technology Faculty Of Engineering University Of Tokus
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藤井 尊
Tag Biosystem Institute
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河 憲
Biological Science and Technology, Faculty of Engineering, University of Tokushima
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濱本 英利
Tag Biosystem Institute
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尹 水弘
College of Pharmacy, Taegu Hyosung Catholic University
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河 憲
Biological Science And Technology Faculty Of Engineering University Of Tokushima:college Of Pharmacy
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尹 水弘
College Of Pharmacy Taegu Hyosung Catholic University
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Yoon Soo
College Of Pharmacy Taegu Hyosung Catholic University
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濱本 英利
Tag Biosystem Institute:biological Science And Technology Faculty Of Engineering University Of Tokus
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Fujii Takeru
徳島大学 工
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藤井 尊
Tag Biosystem Institute:biological Science And Technology Faculty Of Engineering University Of Tokus
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- Applications of MTT Assay to Primary Cultured Rat Hepatocytes
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