Dopingに関する研究(第1報)薄層クロマトグラフィーによるウマ尿中薬物のスクリーニング法

概要

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To obtain the most suitable conditions for thin-layer chromatographic screening procedure for detection of doping drugs, 8 solvents and 9 reagents were compared, using 28 neutral, basic, and acidic drugs. Disturbance of separation by tartaric acid added to urine extracts to prevent loss of drugs by evaporating is proved to be eliminated by over-spotting of concentrated NH_4OH on the original line before running. The method elaborated in the present investigation is as follows : The methanol solution of the residue from chloroform extract, corresponding to 8 ml of urine, containing 2 mg of tartaric acid, is spotted on silica get plate containing a fluorecent substance. After over-spotting of concentrated NH_4OH, the plate is developed with chloroform -methanol (9 : 1). The spots of drugs are visualized by the following means : (1) Ultraviolet irradiation, (2) 1% HgNO_3,(3) Mandelin's reagent, (4) Dragendorff's reagent. Urine samples must be frozen when analysis is not done immediately. A plate containing mixed fluorecent substances is suitable for this method. The sensitivity was not inferior to any other method by ultraviolet spectra, color test, and microcrystal test using 200 ml of urine.
公益社団法人日本薬学会の論文
1972-09-25