金属-ヌクレオチド錯体の研究(第3報)5′-アデニル酸-金属錯体の赤外線吸収スペクトルおよびNMRスペクトルによる研究

概要

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The insoluble metal-5'-AMP complexes were isolated and the metal coordination sites on 5'-AMP were investigated by infrared absorption and nuclear magnetic resonance (NMR) spectra. Spectral changes in complex formation were discussed on the basis of absorption bands of the adenine ring moiety (1700-1600 cm^<-1>) and phosphate groups (1200-900 cm^<-1>) in comparison with those of 5'-AMP and 5'-AMP sodium salt which were prepared from various pH solutions. Shifts of C=N^+ at 1690 cm^<-1> to 1705 cm^<-1> and 1675 cm^<-1> in complexation with Pb^<2+> and Cd^<2+>, respectively, and a little change in 1200-900 cm^<-1> showed that the adenine ring moiety and also the phosphate group are the sites of coordination of these metals. Other metals such as Fe^<3+>, Al^<3+>, UO_2^<2+>, ZrO^<2+>, and Ag^+ coordinate mainly the phosphate group of 5'-AMP. The coordination of Pb^<2+> and Cd^<2+> to the adenine ring moiety was confirmed clearly by NMR spectra, in DMSO-d_6 but the site of coordination in the adenine ring could not be determined.
公益社団法人日本薬学会の論文
1971-06-25