血中および尿中のピンドロールの高速液体クロマトグラフィーによる定量

概要

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A high pressure liquid chromatography (HPLC) by use of ultraviolet absorption detector was examined for the determination of pindolol in the plasma and urine. The pindolol extracted from biological fluid and the disopyramide used as an internal standard, were chromatographed as ion pairs with heptanesulphonic acid by use of Nucleosil 7C_<18> reverse phase column. The coefficients of variation for the peak height ratio were 2.7% (50 ng/ml) to 4.3% (10 ng/ml) in the plasma and 2.3% (20 μg/ml) to 3.8% (5 μg/ml) in the urine. The detection limits of pindolol were about 2 ng/ml in the plasma and 0.5 μg/ml in the urine. The plasma levels and the urinary excretion rates of pindolol after oral administration of 10 mg pindolol to a healthy male volunteer could be measured by this method. This method is more sensitive and reproducible than the fluorometric method and may be used for a clinical pharmacokinetic study and a bioavailability study of pindolol.
公益社団法人日本薬学会の論文
1981-02-25