マウス脾細胞を用いた Staphylococcal Phage Lysate (SPL) の抗体産生能に及ぼす影響

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The effects of staphylococcal phage lysate (SPL) on polyclonal antibody secretion, and mitogenic activity of mice spleen cells were studied. 1) SPL, at a concentration of 30 μl/ml augmented mitogenic activity 1.9 times in mouse spleen cell cultures, however no enhancement of mitogenic activity was observed in B cell depleted, T cell cultures and in athymic nude mouse spleen cell cultures. 2) Augmentation of polyclonal antibody secretion by SPL was observed in athymic nude mouse spleen cell cultures, and did not enhance depleted of macrophages, by passage through Sephadex G-10. 3) By injection of 100 μl SPL, in vivo adjuvant activity against sheep red blood cells were augmented 2.4 times. As revealed above, SPL enhance both mitogenic activity and polyclonal antibody secretion of mouse spleen cells, but augmentation of mitogenic activity is not observed by SPL in T cell, or B cell cultures alone, existence of both T cell and B cell is required to mitogenic activity by SPL. Augmentation of polyclonal antibody secretion by SPL needs B cell, T cell and macrophages.
公益社団法人日本薬学会の論文
1985-08-25

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