アンモニア電極と精製Factor Cを用いたエンドトキシンの定量法

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Factor C, an endotoxin-sensitive serine-protease zymogen, was prepared by cation-exchange chromatography using SP Toyopearl 650C. This preparation exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The endotoxine-dependent Factor C activity was determined from the amount of released ammonium ions by cleavage of N-tert-butoxy-carbonl Val-Pro-Arg-NH_2 using an ammonia electrode. The sensitivity towards the endotoxin was approximately 5×10^<-8> g/ml.
公益社団法人日本薬学会の論文
1992-12-25