HPLCを利用したヒト全血, 血漿及び尿試料中フェノフィブリン酸及びその還元体の定量

概要

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In this study, a very reliable HPLC method was developed for the determination of fenofibric acid and reduced fenofibric acid in the biological samples described as follows. After addition of the internal standard solution and 0.5M HCl to the biological sample, fenofibric acid, reduced fenofibric acid and the internal standard were extracted with a mixed solvent of n-hexane and ethyl acetate (90 : 10) from the mixture. The acids were back-extracted from the organic phase with 0.1M Na_2HPO_4 and then re-extracted from the aqueous phase with a mixed solution of n-hexane and ethyl acetate (95 : 5) after addition of 0.5M HCl. The organic phase was evaporated to dryness under the vacuum. The residue was dissolved in MeOH and diluted with distilled water. An aliquot of the resulting solution was injected on to the HPLC. High reproducibility was observed in this HPLC method (C. V.% less than 4%). Moreover it was confirmed that the conjugates in the urine could be hydrolyzed by incubation at 37℃ for 18h after addition of 400IU of β-glucuronidase.
公益社団法人日本薬学会の論文
1998-10-01