安定同位体標識化ヌクレオシド類の効率的合成法の開発並びにそれらの構造生物学的利用

概要

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1) A series of synthetic works on nucleosides appropriately labeled with stable isotopes of deuterium, carbon-13,and nitrogen-15 has been undertaken, confronting the strong demands for the nucleosides in the NMR spectroscopic study deeply related to structural biology, and the synthetic methods of (2'R)-and (2'S)-2'-deoxy[2'-^2H]ribonucleosides, 2'-deoxy[5'-^2H]ribonucleosides, [5'-^<13>C]ribonucleosides, 7'-deoxy[5'-^13C]ribonucleosides, 2'-deoxy[4-^<15>N]cytidine, [4-^<15>N]cytidine, 2'deoxy[6-^<15>N]adenosine, and [6-^<15>N]adenosine were developed more efficiently than ever; some oligodeoxyribonucleotides were constructed by the use of these materials and found to be extraordinarily feasible for the NMR spectroscopic studies. 2) A novel approach to oligonucleotide systhesis on CPG has been established by the use of a base-labile protecting group, i.e., 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) protecting group for the 5'-hydroxyl group of nucleoside 3'-phosphoramidites.
公益社団法人日本薬学会の論文
1999-05-01