特異的抗血清を用いるラジオイムノアッセイによる血漿中 Dihydroergotoxine の測定

概要

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Dihydroergotoxin (DET)-bovine serum albumin (BSA) conjugate in which the hapten molecule is linked to BSA through the N-1 position on the DET, was prepared by means of the Mannich reaction. Antisera raised against DET-[N-1]-BSA immunogen in guinea pigs and rabbits possessed excellent specificity to DET, exhibiting no significant cross-reactions with dihydroergotamine and the metabolite of dihydroergocristine (<1%). The use of specific antiserum and radioactive tracer (^3H-dihydro-α-ergocryptine, 42 Ci/mmol) allowed to assay DET in the plasma (detection limit ca. 10 pg/ml) directly without extraction. The proposed assay system was applied for measuring the plasma DET level in dogs after oral administration of a tablet of DET mesylate(2 mg).
公益社団法人日本薬学会の論文
1988-01-25