ガスクロマトグラフィーによる尿中ニコチン, コチニンの同時定量法について

概要

論文の詳細を見る
A rapid and sensitive method for the simultaneous determination of nicotine and its principal metabolite, cotinine in the urine is described. An Extrelut column extraction procedure was introduced and the quantitative analyses were performed by gas chromatography equipped with a thermionic detector. This method had a good reproducibility and the recovery approximately 100 (93-102) per cent. Urinary and plasma cotinine levels determined by our method were compared with those determined by radioimmunoassay. There was a good correlation between them. There was a highly significant difference in urinary nicotine level, nicotine/creatinine ratio, cotinine level and cotinine/creatinine ratio between nonsmokers and smokers. Cigarettes consumed per day highly correlated with urinary cotinine level and cotinine/creatinine ratio, but not with urinary nicotine level and nicotine/creatinine ratio.
公益社団法人日本薬学会の論文
1986-08-31