糞中ステロイドの分析による人畜異同識別

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概要

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• The study aims at demonstration of fecal pollution indicator by analysis of the steroids found in human, pig, and cattle feces. The chloromethyldimethylchlorosilane (CMDS)-derivatives of the fecal stanols were specifically separated on Florisil column chromatography by elution with n-hexane containing 0.5% ethyl ether (Fraction 1). GC analysis revealed that Fraction 1 had mainly four components, named as Peak (1)-Peak (4). These components were subsequently identified as coprostanol (Peak (1)), and tentatively as methylcoprostanol (Peak (2)), 24-ethyl-5β-cholesten-3β-ol (Peak (3)), ethylcoprostanol (Peak (4)) by mass fragment spectrometry (GC-MS). It was found that the peak height ratio (Peak (4)/Peak (1)) was characteristically different among animal species, suggesting useful indicator of fucal pollution. Mean values of the ratio were given as 0.167 (γ, 0.767 ; n, 40) for human, 0.569 (γ, 0.703 ; n, 45) for pig, 1.33 (γ, 0.944 ; n, 32) for cow, 1.65 (γ, 0.651 ; n, 10) for ox, and 1.12 (γ, 0.974 ; n, 10) for Japanese cattle.

• 公益社団法人日本薬学会の論文
• 1986-04-30

著者

• 本多 正夫

  新潟県警科学捜査研究所

• 篠原 利明

  科学警察研究所, 化学第二研究室

関連論文

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• 糞特有ステロイドの分析による屎尿汚染の鑑別例と人畜汚染指標に与える配合飼料中のステロイドの影響について
• 糞中ステロイドの分析による人畜異同識別
• 再製紙スラッジに残存するPCBの溶出試験についての検討

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