Thin-Layer Chromatography with Flame Ionization Detector for Determination of Methomyl in Serum and Urine Using a Rapid Sep Pak Cartridge Extraction

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A thin-layer chromatographic method with flame-ionization detector for the quantitative evaluation of methomyl (S-methyl N-[(methylcarbamoyl) oxy] thioacetimidate) in serum and urine is described. Methomyl in biological fluids was separated and purified simply and rapidly by passing the samples through a Sep Pak C_<18> or Silica cartridge, in contrast to the complicated pretreatment required in conventional methods. The detection limit was 0.07 μg in the spotted volume using Chromarod A with acetone-hexane (1 : 4,v/v) as the solvent system. The recoveries were 104.0% in serum, and 98.5% in urine. Further, the method showed good precision ; the coefficient of variation was 2.4% in serum, and 4.0% in urine. The reproducibility was satisfactory, and this method could be performed far more simply and rapidly than the conventional ones.
公益社団法人日本薬学会の論文
1985-04-30