高速液体クロマトグラフィーによるトルエン吸入者の尿中馬尿酸の定量

概要

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Hippuric acid in the human urine was determined by high-performance liquid chromatography with a UV detector by use of stainless column (4.0 mm×0.15 m) packed with Nucleosil _5C_<18>. Mobile phase was H_2O-methanol (80 : 20), adjusted to pH 3.0 with H_3PO_4. The sample solution was extracted with ethyl acetate after adjusting to pH 1.0 with 6N HCl. Determination was carried out successfully by using vanillic acid as an internal standard and by maintaining the temperature at 40°. The recovery of hippuric acid from aqueous solution (0.5 mg/ml) was 96.0±1.5%. The contents of hippuric acid detected in samples from suspected persons of toluene inhalation were 4.83,16.01,2.48 and 6.41 mg/ml, respectively.
公益社団法人日本薬学会の論文
1981-04-30