環境汚染物質に対するゲルクロマトグラフィーの応用 : 土壌中のベンゾ〔α〕ビレンの定量

概要

論文の詳細を見る
Benzo [α] pyrene in soil was determined by gel chromatography and spectrofluorometry with good results in terms of simplicity, reproducibility, and accuracy. Benzo [α] pyrene in the soil samples was extracted with 120 ml of benzene-methanol (5 : 1) mixture in a Soxhlet apparatus and then clean-up was made by alumina (moisture content 7% (v/w)) column chromatography with hexane. Benzo [α]-pyrene was fractionated from this extract by Sephadex LH-20 column (1×25 cm) using isopropanol as the mobile phase. Effluent was collected in 5 ml fractions over the range of 35 to 50 ml, and benzo [α]-pyrene was determined by spectrofluorometry, by the base line method. Benzo [α] pyrene content in the soil determined by this method was 1.3-17.9 ppb in hilly and seaside areas, 107-486 ppb in urban area with extremely heavy traffic, and 32 ppb in the control area with light traffic. No apparent correlation was found between particle size of soil and distribution rate of benzo [α] pyrene.
公益社団法人日本薬学会の論文
1978-12-31