過ヨード酸-チオバルビツール酸法によるストレプトマイシンの比色定量

概要

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For the purpose of establishing a sensitive method for the determination of streptomycin in organic materials, the colorimetric method reported by Duda was investigated and the two main procedures in the original method were modified. With this modified method, 2.5-60 μg/ml of streptomycin in water was successfully determined and sensitivity of the method was twice that of the original method. The effect of protein coexisting with streptomycin on the colorimetry was examined. In lower concentration, protein suppressed the color development, and the absorbance in 1.5 mg/ml of albumin solution decreased the most and was as low as 80% of the control. On the contrary, in higher concentration of more than 6 mg/ml, protein promoted the reaction and the absorbance in 16 mg/ml of albumin solution was as high as 136% of the control. Thorough elimination of protein from the sample is therefore necessary to obtain an accurate streptomycin value in organic materials, but the technique is not yet established.
公益社団法人日本薬学会の論文
1976-12-31