Mutagenicity of an Ozonized Solution of Phenylenediamine Derivatives in the Salmonella Test

概要

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o-Phenylenediamine (o-PD), m-phenylenediamine (m-PD), 2,4-diaminotoluene (DAT) or 2,4-diaminoanisole (DAA) in aqueous solutions were oxidized with ozone at pH 10.7,and the ozonized solutions were tested for their mutagenicity in Salmonella typhimurium TA 98 in the presence or absence of a mammalian metabolic activation system (S 9 mix). The mutagenicity of m-PD and DAA aqueous solutions with S 9 mix was markedly enhanced by oxidation with 1-to 4-fold mol of ozone, but vanished with sufficient ozonation. An aqueous solution of m-PD and DAA was ozonized at pH 4.0,7.0 and 10.7,and the mutagenicity of the ozonized solution and extract with ethyl acetate (AcOEt) was tested in S. typhimurium TA 98 with S 9 mix. At all pH values of the solution, mutagenicity was markedly enhanced by ozonation, and that of the AcOEt extract was equal to or greater than that of the aqueous solution. Aminophenazines were observed in the AcOEt extract from the ozonized solution of m-PD and DAA. The contribution of the aminophenazines to mutagenicity, however, was low, and it is suggested that other major mutagenic compounds were formed.
1997-12-31