Cytotoxicity of Trihalomethanes and Lipid Peroxidation in Isolated Rat Hepatocytes

概要

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Cytotoxic and lipid peroxidative effects of 4 major trihalomethanes (chloroform, CHCl_3 ; bromodichloromethane, BrCHCl_2 ; chlorodibromomethane, ClCHBr_2 ; bromoform, CHBr_3) were examined in isolated rat hepatocytes. The phospholipid hydroperoxides, phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH), were determined by the HPLC-chemiluminescence detection (CL-HPLC) method which is sensitive and specific for lipid hydroperoxide. Severe cytotoxicity was observed when the cells were cultured with 50 mM of CHCl_3,20 mM of BrCHCl_2,10 mM of ClCHBr_2 and 10 mM of CHBr_3,respectively. At these concentrations, cytotoxicity was observed after 10 min or more incubation with each 4 trihalomethane. The cellular lipid hydroperoxide was remarkably increased with the cytotoxicity by BrCHCl_2 and the other three trihalomethanes induced slight lipid peroxidation. The cytotoxicity was not effectively prevented by α-tocopherol, though the lipid peroxidation was appreciably prevented. These results suggested that trihalomethane induces lipid peroxidation in isolated rat hepatocytes, but that this may not be the main cause of the cytotoxicity.
社団法人日本薬学会の論文
1996-12-31