Cytokine Production by Human Peripheral Blood Mononuclear cells after Exposure to Heavy Metals

概要

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The effects of heavy metal exposure on the immune system were determined by measuring cytokine production of human peripheral blood mononuclear cells (h-PBMCs) from a healthy female. The h-PBMCs were exposed for 3d to CdSO_4,K_2Cr_2O_7,and HgCl_2 at 1,5,10μм and to (CH_3COO)Pb at 10,50,and 100μм. Concentrations of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β, IL-6,IL-8,and IL-10 were quantified using the ELISA method. The results showed that cytokines assayed were differentially affected by heavy metal exposure. Chromium significantly increased the production of IL-1β while decreasing the production of IFN-γ, IL-6,IL-8 and IL-10. Mercury at low concentrations increased the levels of TNF-α and IL-1β. At higher concentrations, an opposite effect was seen. Cytokines may function as biomarkers in elucidating the mechanisms of the immunotoxic effects of heavy metals.
公益社団法人日本薬学会の論文
2000-10-01