Short-Term Screening Method for the Prediction of Carcinogenicity of Chemical Substances : Current Status and Problems of an in vivo Rodent Micronucleus Assay
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概要
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Various short-term screening methods have been developed to detect mutagenic/carcinogenic substances. They have played important roles not only in screening suspected chemicals but in studying the mechanisms of mutagenesis/carcinogenesis, and have provided useful information for assessing the genetic effects of chemicals on humans. The micronucleus assay is an in vivo mammalian methods, which has been widely used for screening the genotoxic potency of chemical substances. By this assay, the genotoxicity of chemicals, including clastogens and spindle poisons, have been evaluated using immature bone marrow erythrocytes in mice. The use of immature erythrocytes in circulating peripheral blood instead of in bone marrow of mice has been developed in recent years and it has been determined that it gives results as relevant as those of bone marrow cells in mice. Using the peripheral blood of mice has made the micronucleus assay method more practical and useful. Recently, rats have been identified as an acceptable species for this assay, and assays using the peripheral blood of rats are often carried out concomitantly with general toxicity or carcinogenic studies. However, it must be noted that the micronucleus assays have some limitations. The activity of substances which are metabolized rapidly in mice or rats, for example, may hardly be detected by the micronucleus assay; metabolic features of the substances may affect the assay results greatly. In this review, the current status of the micronucleus assay is discussed as a short-term screening method in the context of its usefulness and its limitations.
- 公益社団法人日本薬学会の論文
- 2001-02-01
著者
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Tomita Isao
Laboratory Of Health Science Shizuoka College Of Pharmaceutical Sciences
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Tomita Isao
Laboratory Of Life Science Shizuoka Sangyo University
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Sato Sei-ichi
Japan Tobacco Inc., Toxicology Research Laboratories, Central Pharmaceutical Research Institute
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Sato Sei-ichi
Japan Tobacco Inc. Toxicology Research Laboratories Central Pharmaceutical Research Institute
関連論文
- Detection of Mutagenic Compounds in Areas Inhabited by Nibea mitsukurii and Their Tumor-Inducing Effects in Fish (Proceedings of the 12 th Symposium on Environmental Pollutants and Toxicology) (Regular Presentations)
- Detection and Isolation of Mutagenic Compounds from Sea Water. III. Seasonal Changes of Mutagenic Activities and Physico-chemical Indexes of Sea Water (Regular Presentations) (Proceedings of the 10 th Symposium on Environmental Pollutants and Toxicology)
- Detection and Isolation of Mutagenic Compounds from Sea Water (II) (Regular Presentations) (Proceedings of the 9th Symposium on Environmental Pollutants and Toxicology)
- DETECTION AND ISOLATION OF MUTAGENIC SUBSTANCES FROM SEA WATER
- MUTAGENIC SUBSTANCES FOUND IN THE AQUATIC ENVIRONMENT AS RELATED TO THE NEOPLASMS IN NIBEA MITSUKURII
- Short-Term Screening Method for the Prediction of Carcinogenicity of Chemical Substances : Current Status and Problems of an in vivo Rodent Micronucleus Assay
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