Cell-Density-Dependent Expression of the β-Adrenergic Response by Epidermal Growth Factor (EGF) in Primary Cultures of Adult Rat Hepatocytes

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概要

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• The effects of epidermal growth factor (EGF) and cell density of the apperance of β-adrenergic responses were exmianed in primary cultures of adult rat hepatocytes. The β-adrenergic response was measured as the ability to accumulate cAMP by β_2-agonist metaproterenol in monolayers that had been cultured without or with 20 ng/ml EGF. Hepatocytes cultured with EGF at a high ell density (1.0×10^5 cells/cm^2) showed a relatively lower response to 10 μM metaproterenol. In contrast, when cultured at a low cell density (3.3×10^4 cells/cm^2) with EGF, the cells showed a higher response to the β-adrenergic agonist. These responses were blocked by the β-adrenergic antagonist propranolol (10 μM). The β-adrenergic response increased rapidly with culture time. The addition of cycloheximide (5 μM) to the culture abolished the expression of β-adrenergic response. The enhanced β-adrenergic response by 20 ng/ml EGF was partially inhibited by the addition of cytochalasin B (20 μM) to the culture. The cAMP-producing response to metaproterenol (10 μM) was dose-dependently inhibited by the specific α_2-agonist UK-14304. Pretreatment of the hepatocytes with pertussis toxin (100 ng/ml) potentiated the β-adrenergic response. These results demonstrate that augmented β-adrenergic responsiveness can be acquired by adult rat hepatocytes cultured with 20 ng/ml EGF at a low cell density, and the β-adrenergic response involves de novo synthesis of protein(s). The results also show that significant α_2- and β-adrenergic rsponses coexist in the primary cultures of adult rat hepatocytes.

• 公益社団法人日本薬学会の論文
• 1996-05-15

著者

• Ogihara Masahiko

  Biochemical Pharmacology Group Faculty Of Pharmaceutical Sciences Josai University

関連論文

• Proliferation of Adult Rat Hepatocytes in Primary Cultures Induced by Platelet-Derived Growth Factor Is Potentiated by Phenylephrine
• Cell-Density-Dependent Expression of the β-Adrenergic Response by Epidermal Growth Factor (EGF) in Primary Cultures of Adult Rat Hepatocytes
• Cell-Density-Dependent Expression of the α_2-Adrenergic Response by Epidermal Growth Factor (EGF) in Primary Cultures of Adult Rat Hepatocytes
• Expression of α_2- and β_2-Adrenergic Responses by Hepatocyte Growth Factor and Platelet-Derived Growth Factor in Primary Cultures of Adult Rat Hepatocytes
• Expression of α_2 - Receptor - Mediated Responses by Insulin in Primary Culture of Rat Hepatocytes

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