Isolation and Characterization of a cDNA Encoding a Human TFIID Subunit Containing a Variety of Putative Structural Motifs Including Direct Repeats
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA encoding human TFIID subunit p30β (hp30β) was isolated and sequenced. Comparison of the deduced 211 amino acid (aa) sequence with that of Drosophila melanogaster p30β (dp30β) showed 45% identity in overall length, and subunits with highly conserved C-terminal (81% identity) and less conserved N-terminal (21% identity) regions. Three acidic and basic regions were alternately placed. Potent target sites for protein kinases and a putative nuclear localization signal (NLS) were located in the N-terminal region. Interestingly, analysis of the aa sequence of p30β led us to find several interesting structural motifs/regions including 1) direct repeats, 2) a region similar to histone H4 between the two direct repeats and 3) acidic and hydrophobic aa surfaces in the potent helical region. Northern blot analysis showed ubiquitous expression of mRNA corresponding to hp30β.
- 公益社団法人日本薬学会の論文
- 1996-01-15
著者
-
KUZUHARA Takashi
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
-
Kuzuhara T
Univ. Tokyo Tokyo
-
Horikoshi M
Univ. Tokyo Tokyo
-
Horikoshi Masami
Laboratory Of Developmental Biology Department Of Cellular Biology Institute Of Molecular And Cellul
関連論文
- Generation of Mouse Monoclonal Antibody against (-)-Epigallocatechin Gallate(Biochemistry)
- Presence of a Motif Conserved between Helicobacter pylori TNF-α Inducing Protein (Tipα) and Penicillin-Binding Proteins(Miscellaneous)
- Restricted Expression of a Member of the Transcription Elongation Factor S-II Family in Testicular Germ Cells during and after Meiosis^1
- The Involvement of the Histone Fold Motifs in the Mutual Interaction between Human TAF_80 and TAF_22
- A Decade of Histone Acetylation : Marking Eukaryotic Chromosomes with Specific Codes
- Isolation and Characterization of a cDNA Encoding a Human TFIID Subunit Containing a Variety of Putative Structural Motifs Including Direct Repeats
- Relationship between the Subcellular Localization and Structures of Catalytic Domains of FKBP-Type PPIases