Purification and Characterization of PK-120,a Novel Substrate for Plasma Kallikrein, from Guinea Pig Plasma

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概要

• 論文の詳細を見る

• PK-120 is a novel substrate of plasma kallikrein isolated from human plasma. Western blotting using anti-human PK-120 polyclonal antibodies demonstrated the presence of a PK-120-like protein in guinea pig, rat and mouse plasma. We purified the immunoreactive-120 kDa protein from guinea pig plasma by polyethylene glycol fractionation followed by ion-exchange chromatography using Q-Sepharose, heparin-Sepharose, Mono-Q and gel filtration with TSK G3000. The 120 kDa protein thus isolated was similar to human PK-120 with respect to limited proteolysis by kallikrein, amino acid composition, and the N-terminal amino acid sequence, indicating that the immunoreactive 120 kDa protein was guinea pig PK-120.

• 公益社団法人日本薬学会の論文
• 1995-06-15

著者

• Pu Xiao

  Department Of Hygienic Chemistry Faculty Of Phyarmaceutical Sciences Hokkaido University

• 長澤 滋治

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokkaido University

• 蒲 小平

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokkaido University

関連論文

• Purification and Characterization of PK-120,a Novel Substrate for Plasma Kallikrein, from Guinea Pig Plasma

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