Effect of Divalent Metal Ions on the Binding of Thyroxine to Bovine Serum Albumin as Measured by Fluorescence
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概要
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The binding of thyroxine (T_4) to bovine serum albumin (BSA) has been studied in the presence and absence of Ca^<2+>, Cu^<2+> and Zn^<2+> ions at various pH's in 0.1 M Tris-acetate buffer at 25℃ using the fluoresence method. In the presence of 50 μM Ca^<2+> and Zn^<2+> and the absence of metal ions, the binding constant (K) increased similarly with increasing pH values from pH 5 to pH 9,and the K value near midpoint, pH 7.4,was 1.66±0.17×10^6 M^<-1>. By contrast, the binding constant remained constant between pH 5 and pH 9 in the presence of 10 μM Cu^<2+>, with an average value of 1.61±0.22×10^6 M^<-1>, suggesting a significant influence of Cu^<2+> ions on T_4 binding to BSA.
- 公益社団法人日本薬学会の論文
- 1993-07-15
著者
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岡部 亘雄
Faculty of Pharmaceutical Sciences, Kinki University
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岡部 亘雄
Faculty Of Pharmaceutical Sciences Kinki University
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帆風 美佐
Faculty of Pharmaceutical Sciences, Kinki University
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帆風 美佐
近畿大学薬学部
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