Production and Characterization of Monoclonal Antibody That Simultaneously Recognizes Methamphetamine and Its Major Metabolite

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概要

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• A series of monoclonal antibodies (mAbs) that react with methamphetamine-bovine serum albumin (MA-BSA) were established by intrasplenic immunization method. Among established 36 clones, two typical mAbs, designated NK-1 and NK-2,were described. The inhibition assay of enzyme-linked immunosorbent assay (ELISA) analysis using methamphetamine analogs indicated that NK-1 showed considerable reactivity not only MA-BSA but also methamphetamine and its major metabolite, para-hydroxymethamphetamine (p-hydroxymethamphetamine). The cross-reactivity between NK-1 and the methamphetamine analogs with modified alkyl side chain, indicates that methyl groups of R_5 and R_7 in the methamphetamine molecules are important for the maximum affinity. The length of alkyl side chain on methamphetamine significantly affected the binding affinity of NK-1. The results may suggest that NK-1 will recognize not only methamphetamine but also the bridge part of the methamphetamine that binds the methamphetamine molecules to a carrier protein.

• 公益社団法人日本薬学会の論文
• 1993-05-15

著者

• 南 景〓

  Laboratory Of Immunochemistry Genetic Engineering Research Institute Korea Institute Of Science And

• 崔 明子

  Doping Control Center Korea Institute Of Science And Technology

• 金 載和

  Laboratory of Immunochemistry, Genetic Engineering Research Institute, Korea Institute of Science an

• 韓 美暎

  Laboratory of Immunochemistry, Genetic Engineering Research Institute, Korea Institute of Science an

• 崔 仁成

  Laboratory of Immunochemistry, Genetic Engineering Research Institute, Korea Institute of Science an

• 鄭 兌和

  Laboratory of Immunochemistry, Genetic Engineering Research Institute, Korea Institute of Science an

• 崔 仁成

  Laboratory Of Immunochemistry Genetic Engineering Research Institute Korea Institute Of Science And

• 鄭 兌和

  Laboratory Of Immunochemistry Genetic Engineering Research Institute Korea Institute Of Science And

• 韓 美暎

  Laboratory Of Immunochemistry Genetic Engineering Research Institute Korea Institute Of Science And

• 金 載和

  Laboratory Of Immunochemistry Genetic Engineering Research Institute Korea Institute Of Science And

関連論文

• Production and Characterization of Monoclonal Antibody That Simultaneously Recognizes Methamphetamine and Its Major Metabolite

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