Effect of Cupric Ion on Cholesteryl Ester Hydrolysis in Rat Peritoneal Macrophages

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概要

• 論文の詳細を見る

• The inhibitory effects of Cu^<2+> on the hydrolysis of cholesteryl [4-^<14>C]oleate droplets in rat peritoneal macrophages were studied. Macrophages rapidly incorporated cholesteryl [4-^<14>C]oleate droplets and hydrolyzed at a linear rate over 9h.When macrophages were preincubated in the medium containing Cu^<2+>, the hydrolysis of cholesteryl oleate within macrophages was markedly inhibited by Cu^<2+> and eventually led to the accumulation of cholesteryl oleate in macrophages. The inhibitory effect on the hydrolysis of cholesteryl oleate was dependent on the concentration of Cu^<2+>. Also, the activity of acid cholesteryl ester hydrolase (acid CEH) in lysosomal fractions isolated from rat peritoneal macrophages with Cu^<2+> showed a marked decrease. This decrease also occurred in a dose dependent manner.These results suggest that Cu^<2+> inhibits the hydrolysis of cholesteryl ester in macrophages, and that the accumulation of cholesteryl ester in macrophages of the early atheroscleotic lesion is responsible for the decrease of lysosomal acid CEH activity.

• 公益社団法人日本薬学会の論文
• 1993-02-15

著者

• 田中 満男

  Showa College of Pharmaceutical Sciences

関連論文

• Properties of an Albumin Inhibiting Lysosomal Acid Cholesteryl Ester Hydrolase in Rat Liver
• Antioxidant Action of Thiopalmitic Acid on Microsomal Lipid Peroxidation
• Capillary Gas Chromatography Quantification of Cholesterol in Copper-Oxidized Low-Density Lipoprotein
• Effect of Cupric Ion on Cholesteryl Ester Hydrolysis in Rat Peritoneal Macrophages

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