Preparation of Specific Antisera to Digoxin by Using Digoxin C-3' and C-3" Hemisuccinate-Bovine Serum Albumin Conjugates

スポンサーリンク

概要

• 論文の詳細を見る

• The preparation and antigenic properties of digoxin C-3' and C-3" hemisuccinate-bovine serum albumin (BSA) conjugates are described. The antisera were prepared by immunizing rabbits with each of the digoxin-BSA conjugates, and properties of the antisera were characterized by RIA with ^3H-labeled digoxin. The antidigoxin antiserum from immunization with digoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digoxin, exhibiting fairly low cross-reactions with dihydrodigoxin (2.1%), digoxigenin monodigitoxoside (0.9%), digoxigenin bisdigitoxoside (0.6%), and digoxigenin (0.1%).

• 2000-08-01

著者

• Fujii Youichi

  Faculty Of Pharmaceutical Sciences Hokuriku University

• Ikeda Yukari

  Faculty Of Pharmaceutical Sciences Hokuriku University

関連論文

• Development of Radioimmunoassay for Measurement of Serum Digoxin in Digitalized Patients Using Novel Anti-digoxin Antiserum
• Determination of digoxin in human serum using stable isotope dilution liquid chromatography/electrospray ionization-tandem mass spectrometry
• Properties of Novel Anti-digoxin Antisera in Radioimmunoassay Using Homologous and Site Heterologous Tritium-Labeled Antigens Involving a [^3H]-Leucine Moiety(Analytical Biochemistry)
• Preparation of Specific Antisera to Digoxin by Using Digoxin C-3' and C-3" Hemisuccinate-Bovine Serum Albumin Conjugates

スポンサーリンク