Study of the β_1 Adrenergic Receptor Expression in Human Tissues : Immunological Approach

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概要

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• Questions exist regarding tissue distribution of the β_1 adrenergic receptor (β_1-AR). The aim of this study was to investigate relative distribution patterns of the β_1-AR at the protein level in a variety of human tissues by Western blot analysis. The specificity of anti-peptide antibodies was confirmed both by Western blot with recombinant β_1-AR expressed as a membrane protein in E. coli and by immunoprecipitation of membranes from Sf9 cells infected with baculovirus to express the human recombinant β_1-AR. β_1-AR was found in all tissues examined. The relative amount of protein varied significantly between the tissues, from highest in lung and testis to very low in liver. β_1-ARs were rather abundant in heart, kidney, placenta, spleen and thyroid. These results reveal unique distribution of β_1-AR protein that suggests its tissue specific role. Moreover, our data demonstrate a high sensitivity of immunological detection that allows direct comparison of β_1-AR subtype expression and could be used for receptor study in biopsies available in limited amounts, such as human heart biopsy.

• 公益社団法人日本薬学会の論文
• 2000-06-01

著者

• Hellgren Irina

  Karolinska Institute At Department Of Cardiology Huddinge University Hospital

• Magnusson Yvonne

  Wallenberg Laboratory For Cardiovascular Research Sahlgrenska University Hospital

• SYLVEN Christer

  Karolinska Institute at Department of Cardiology, Huddinge University Hospital

• Sylven Christer

  Karolinska Institute At Department Of Cardiology Huddinge University Hospital

関連論文

• Study of the β_1 Adrenergic Receptor Expression in Human Tissues : Immunological Approach
• β1 and β2 Adrenoceptor Ligand and mRNA Expression in Dilated Cardiomyopathy
• Na, K-ATPase Receptor Subunits α1,α2 and α3 mRNA in Dilated Cardiomyopathy

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