Authentication of an Animal Crude Drug, Zaocys, by Diagnostic PCR

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概要

• 論文の詳細を見る

• A pair of diagnostic primers for distinguishing the Chinese crude drug Zaocys (Zaocys dhumandes) from its substitutes was designed based on the sequence data of the original animal of the drug and substitutes. Total DNAs were extracted from genuine crude drug and 5 of its substitutes, as well as from 12 species of original animal of the snake crude drug. Diagnostic PCRs were performed using the primers with these total DNAs as a template, annealing at 60-65℃. Positive amplifications were obtained from all DNA templates of Zaocys, whereas negative amplifications were obtained from that of others. The results indicate that Zaocys samples could be definitely distinguished from its substitutes by diagnostic PCR, and no incorrect discrimination was found under the same reaction condition. The advantages of the method in the authenticaiton of crude drugs are also discussed in the present paper.

• 公益社団法人日本薬学会の論文
• 2000-05-01

著者

• Wang Yiquan

  Institute Of Genetic Resources Nanjing Normal University:department Of Biology And Biotechnology Res

• TSIM Karl

  Department of Biology, The Hong Kong University of Science and Technology

• Tsim Karl

  Department Of Biology And Biotechnology Research Institute

• ZHOU Kaiya

  Institute of Genetic Resources, Nanjing Normal University

• XU Luoshan

  Traditional Chinese Pharmaceutical College, China Pharmaceutical University

• DONG Tina

  Department of Biology and Biotechnology Research Institute

• Zhou Kaiya

  Institute Of Genetic Resources Nanjing Normal University

• Xu Luoshan

  Traditional Chinese Pharmaceutical College China Pharmaceutical University:department Of Biology And

関連論文

• Cloning and Characterization of the yjeA Gene, Encoding a Novel Deoxyribonuclease, from Bacillus subtilis
• Authentication of an Animal Crude Drug, Zaocys, by Diagnostic PCR

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