Antioxidant Effects of Albumin-bound Sulfur in Lipid Peroxidation of Rat Liver Microsomes
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概要
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The antioxidant potential of albumin-bound sulfur (SBA) was investigated in rat liver microsomes using lipid peroxidation systems in vitro. Sulfur bound to protein is a reduced metabolite which is produced from cystine by γ-cystathionase. Lipid peroxidation was induced either chemically by ferrous ions and ascorbate or enzymatically by carbon tetrachloride or tert-butyl hydroperoxide as indicated by the increase in thiobarbituric acid reactive substances (TBA-RS) and oxygen consumption. Although the antioxidant effect of SBA was weak on the non-enzymatic lipid peroxidation system, the addition of SBA significantly inhibited TBS-RS formation and oxygen consumption compared with non-treated bovine serum alubumin (BSA) in a microsomal lipid peroxidation system induced enzymatically.The sulfur bound to albumin disappeared during incubation with liver microsomes. However, slight differences in the disapperance were observed depending on whether or not lipid peroxidation was induced in the enzymatic systems. In the CCl_4-induced lipid peroxidation system, the cytochrome P-450 level was significantly decreased by the addition of SBA. Therefore, in cytochrome P-450 dependent lipid peroxidation system, the potential effects of sulfur bound to albumin are due to an inhibition of cytochrome P-450 rather than by the oxidation itself caused by radical trapping.
- 社団法人日本薬学会の論文
- 1999-05-15
著者
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Ogasawara Yuki
Department of Hygienic Chemistry, Meiji Pharmaceutical University
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Ogasawara Yuki
Department Of Environmental Biology Meiji Pharmaceutical University
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ISODA Shinji
Department of Environmental Biology, Meiji Pharmaceutical University
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TANABE Shinzou
Department of Environmental Biology, Meiji Pharmaceutical University
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Isoda S
Department Of Hygienic Chemistry Meiji College Of Pharmacy
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磯田 伸二
Department Of Hygienic Chemistry Meiji College Of Pharmacy
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Isoda Shinji
Department Of Environmental Biology Meiji Pharmaceutical University
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Tanabe S
Department Of Environmental Biology Meiji Pharmaceutical University
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Tanabe Shinzou
Department Of Environmental Biology Meiji Pharmaceutical University
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