S-(1,2-Dicarboxyethyl)glutathione in Yeast : Partial Purification of Its Synthesizing Enzyme
スポンサーリンク
概要
- 論文の詳細を見る
S-(1,2-Dicarboxyethyl)glutathione (DCE-GS) was found in Saccharomyces cerevisiae, but not in bacterial species nor in a unicellular alga (Acetabularia acetabulm). The enzyme that catalyzes condensation of L-malate and glutathione (GSH) to form DCE-GS was partially purified from baker's yeast. It had a molecular mass of 49 kDa and was monomeric and the K_m values were 2.2 and 1.4 mM for L-malate and GSH, respectively. The enzyme had a pH optimum of 7.5. DCE-GS levels in yeast cells were significantly higher in aerobic cultures than in anaerobic ones. DCE-GS was synthesized in cells cultured between 20 and 35℃.
- 社団法人日本薬学会の論文
- 1999-01-15
著者
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坪井 誠二
就実大学薬学部
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TSUBOI Seiji
Faculty of Pharmaceutical Sciences, Okayama University
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KIYONO Kunihiko
Faculty of Pharmaceutical Sciences, Okayama University
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ONO Bun-ichiro
Faculty of Science and Engineering, Ritsumeikan University
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SAKAUE Takahiro
Senju Pharmaceutical Co., Ltd.
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OGATA Kazumi
Senju Pharmaceutical Co., Ltd.
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Ogata Kazumi
Senju Pharmaceutical Co. Ltd.
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Tsuboi Seiji
Faculty Of Pharmaceutical Sciences Okayama University
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Ono Bun-ichiro
Faculty Of Science And Engineering Ritsumeikan University
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Kiyono Kunihiko
Faculty Of Pharmaceutical Sciences Okayama University
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Sakaue Takahiro
Senju Pharmaceutical Co. Ltd.
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