Studies on the Antiviral Mechanisms of Protein Kinase Inhibitors K-252a and KT5926 against the Replication of Vesicular Stomatitis Virus
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概要
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We investigated the antiviral mechanisms of K-252a, a broad non-speciflic protein kinase inhibitor which was isolated from Nocardiopsis sp. and its derivative (KT5926), against vesicular stomatitis virus (VSV) replication in BHK-21 cells. Although K-252a (5 μM) and KT5926 (15 μM) similarly suppressed the viral primary and secondary transcriptions and genomic RNA synthesis in vivo, the inhibitory mechanisms did not seem to be the same; phosphorylation of the viral NS protein was suppressed by K-252a, which might account for the decreased viral RNA synthesis caused by K-252a. On the other hand, KT5926,being known to preferentially inhibit myosin light chain kinase (MLCK), had little effect on NS protein phosphorylation. Cellular casein kinaes II, which is delieved to be involved in the phosphorylation of the N-terminal side (domain I) of NS protein, was not inhibited at all by KT5926 even at 15 μM under in vitro assay conditions, and was only weakly inhibited by K-252a at 1 to 10 μM. Neither inhibitor seemed to directly affect viral protein synthesis, but affected it indirectly as a secondary effect of reduced viral RNA synthesis. These results suggest that both the KT5926-sensitive and the KT5926-resistant but K-252a-sensitive functions are involved in the essential processes of viral RNA synthesis. The KT5926-sensitive function(s) might not be involved in the NS protein phosphorylation, but may participate in some other way in the process of virus replication. On the other hand, the KT5926-resistant, K-252a-sensitive function(s) are probably involved in NS protein phosphorylation. The possible nature of those functions is discussed.
- 公益社団法人日本薬学会の論文
- 1998-05-15
著者
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Kawai A
Department Of Molecular Microbiology Graduate School Of Pharmaceutical Sciences Kyoto University
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Kawai Akihiko
Department Of Cardiovascular Surgery Senpo Tokyo Takanawa Hospital
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Kim Young-sook
Department Of Molecular Microbiology Faculty Of Pharmaceutical Sciences Kyoto University
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Kim Young-sook
Department Of Molecular Microbiology Graduate School Of Pharmaceutical Sciences Kyoto University
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