Establishment of a Standardized Assay System of Fibronectin Activity Using Fibronectin-Mediated Cell Adhesion
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概要
- 論文の詳細を見る
An in vitro assay of fibronectin (FN) was established based on the adhesion of baby hamster kidney (BHK) cells through the cell-binding domain of FN.Each well of a microtiter plate was coated with samples or various concentrations of standard FN. Bovine serum albumin was further coated to prevent the non-specific adhesion of the cells. Various numbers of BHK cells were plated and incubated. After washing out the non-attached cells, the number of attached cells was measured using neutral red (NR)-staining.The conditions for the assay were optimal when 1×10^5 cells/well were plated and incubated for 90 min. The linear relationship between the concentration of FN coated and the absorbance of NR was observed in the range of 0.1-1.0 μg/ml of FN. The inhibition of cell binding by the peptides containing an Arg-Gly-Asp (RGD) sequence demonstrated that this assay system depended on FN-mediated cell adhesion through the major cell-binding domain.
- 公益社団法人日本薬学会の論文
- 1997-12-15
著者
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Shimizu Masato
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Koga Junichi
Development And Research Laboratories Jcr Pharmaceuticals Co. Ltd.
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Nishida Teruo
Department Of Biomolecular Recognition And Ophthalmology Yamaguchi University School Of Medicine
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Nishida Teruo
Department Of Biomolecular Recognition And Ophthalmology Yamaguchi University Graduate School Of Med
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Shimizu Mitsuo
Jcr
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Shimizu Mitsuo
Development And Research Laboratories Jcr Pharmaceuticals Co. Ltd.
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MINAKUCHI Kazunobu
Development and Research laboratories, JCR Pharmaceuticals Co., Ltd.
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MOON Mina
Development and Research Laboratories, JCR Pharmaceuticals Co., Ltd.,
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SHIRONO Chitose
Development and Research Laboratories, JCR Pharmaceuticals Co., Ltd.,
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NISHIMURA Toyohiko
Development and Research Laboratories, JCR Pharmaceuticals Co., Ltd.,
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Moon Mina
Development And Research Laboratories Jcr Pharmaceuticals Co. Ltd.
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Shirono Chitose
Development And Research Laboratories Jcr Pharmaceuticals Co. Ltd.
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Nishimura Toyohiko
Development And Research Laboratories Jcr Pharmaceuticals Co. Ltd.
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Minakuchi Kazunobu
Development And Research Laboratories Jcr Pharmaceuticals Co. Ltd.
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MINAKUCHI Kazunobu
Development and Research Laboratories, JCR Pharmaceuticals Co., Ltd.,
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