Study on Effects of p-Phenylbenzoic Acid on Change of Sulfation and Glucuronidation in Rats

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概要

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• The effects of p-phenylbenzoic acid (PPBA) on sulfation and glucuronidation was studied in rats. Following intravenous injection of ^<14>C-p-tert-butylphenol (^<14>C-TB, 50 μmol/kg) with PPBA (50 μmol/kg), the sulfation of ^<14>C-TB was decreased by 38.8% and its glucuronidation increased by 62.3%. In a system of isolated hepatocytes, the sulfation of ^<14>C-TB increased and its glucuronidation decreased following the addition of 100 μM of PPBA, suggesting competitive inhibition by the glucuronidation of PPBA. On the other hand, the sulfation of ^<14>C-TB decreased and its glucuronidation increased following the addition of 400 μM of PPBA, which represents the plasma concentration of PPBA in vivo. At this concentration, significant leakage of GOT, GPT and LDH into the medium was observed, whereas, no significant change in the ATP synthesis was noted. Microsomes preincubated with 60 μM of PPBA, which represents the intracellular concentration of PPBA in the liver in vivo, showed no change in the level of glucuronidation of ^<14>C-TB. These results suggest that PPBA in the plasma injures cell membranes and causes a leakage of cytosolic sulfotransferase, whereas UDPGT, embedded in the microsomal membrane, does not leak from the injured cell membrane, resulting in the decrease in sulfation and increase in glucuronidation. Similar in vivo effects on the sulfation/glucuronidation ratio were observed for benzoic acids substituted with benzoyl and phenoxy groups.

• 社団法人日本薬学会の論文
• 2002-05-01

著者

• Nanbo Asuka

  Institute For Cognitive Sciences

• Nanbo Toshio

  Institute of Xenobiotic Analyses

• Nanbo T

  Institute Of Xenobiotic Analyses

関連論文

• Study on Effects of p-Phenylbenzoic Acid on Change of Sulfation and Glucuronidation in Rats
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