A Rapid Extraction Procedure of Human Hair Proteins and Identification of Phosphorylated Species
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概要
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We developed a rapid and convenient extraction procedure of human hair proteins to examine their bio-chemical properties in detail. This procedure is based upon the fact that the combination of thiourea and urea in the presence of a reductant can effectively remove proteins from the cortex part of human hair. The extracted fraction mainly consisted of hard α-keratins with molecular masses of 40-60 kDa, matrix proteins with 12- 18 kDa, and minor components with 110-115 kDa and 125-135 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein phosphorylation in human hair was investigated by immunoblotting with antibodies against phosphoserine, phosphothreonine and phosphotyrosine. We found serine phosphorylation in α-keratins and matrix proteins and threonine phosphorylation in α-keratins. The extraction was also found to be effective when wool, chicken feathers, rat hair and human nails were used as starting materials.
- 公益社団法人日本薬学会の論文
- 2002-05-01
著者
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Arimoto Makoto
Faculty Of Textile Science And Technology Shinshu University
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FUJII Toshihiro
Faculty of Textile Science and Technology, Shinshu University
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Fujii T
Faculty Of Textile Science And Technology Shinshu University
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NAKAMURA Akira
Life Science Research Center Lion Corporation
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TAKEUCHI Keiji
Life Science Research Center Lion Corporation
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Takeuchi K
Life Science Research Center Lion Corporation
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Nakamura A
Life Science Research Center Lion Corporation
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Fujii Toshihiro
Faculty Of Textile Science And Technology Shinshu University
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