De Novo Regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and Quantitative Analysis of Harpagoside, an Iridoid Glucoside, Formed in Aerial and Underground Parts of In Vitro Propagated and Wild Plants by HPLC

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概要

• 論文の詳細を見る

• A protocol for de novo regeneration and rapid micropropagation of Scrophularia yoshimurae (Scrophulariaceae) hss been developed. Multipul shoot development was achieved by culturing the shoot-tip, leaf-base, stemnode and stem-internode explants on Murashige and Skoog (MS) medium supplemented with 4.44μM N^6-benzyladenine (BA) and 1.07μM α-naphthaleneacetic acid (NAA). Stem-node and shoot-tip explants showed the highest response (100%) followed by stem-internode (74.4%) and leaf-base (7.7%) explants. The shoots were multiplied by subculturing on the same medium used for shoot induction. Shoots were rooted on growth regulator-free MS basal medium and the plantlets were transplanted to soil and acclimatized in the growth chamber. The content of harpagoside, a quantitatively predominant iridoid glycoside, in different plant material was determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of harpagoside in the aerial and underground parts of S.yoshimurae was significantly higher than the marketed crude drug (underground parts of Scrophularia ningpoensis).

• 公益社団法人日本薬学会の論文
• 2001-11-01

著者

• Chueh Fu-shin

  Department Of Agronomy Taiwan Agricultural Research Institute

• Kuo Chao-lin

  Chung Hwa College Of Medical Technology:institute Of Chinese Pharmaceutical Science China Medical Co

• TSAY Hsin-Sheng

  Department of Applied Chemistry, Chaoyang University of Technology

• SAGARE Abhay

  Department of Agronomy, Taiwan Agricultural Research Institute

• Tsay Hsin-sheng

  Department Of Agronomy Taiwan Agricultural Research Institute

• Sagare Abhay

  Department Of Agronomy Taiwan Agricultural Research Institute

• Sagare Adhay

  Department of Agronomy, Taiwan Agricultural Research Institute

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• De Novo Regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and Quantitative Analysis of Harpagoside, an Iridoid Glucoside, Formed in Aerial and Underground Parts of In Vitro Propagated and Wild Plants by HPLC
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