Hair Analysis for Pharmaceutical Drugs.I.Effective Extraction and Determination of Phenobarbital, Phenytoin and Their Major Metabolites in Rat and Human Hair

スポンサーリンク

概要

• 論文の詳細を見る

• In order to establish an analytical method for the determination of phenobarbital (PB), phenytoin (PPH) and their hydroxylated metabolites in hair, animal model experiments were performed. Five male dark-agouti pigmented rats, aged 5 weeks, were intraperitoneally and orally administered PB or PPH independently at 25 mg/kg once a day for 5 successive days. The growing back hair was collected 15 d after the first administration. Four typical extraction methods, using NH_4OH-methanol-acetone, TFA-methanol-acetone, 1M sodium hydroxide and proteinase K, were evaluated using the rat hair samples containing PB or PPH. Methanol-acetone-NH_4OH (10:10 :1) was the best extraction method from all aspects, such as high extraction efficiency and low noise. The analytes in the extract were methylated in acetonitrile with 20% tetramethylammonium hydroxide and methyliodide at 70℃ for 10min. After purification with Bond Elut Certify, the methylated products were analyzed by GC-MS. From rat hair, PB, p-hydroxy PB, PPH and p-hydroxy PPH were detected at average concentrations of 26.9, trace, 4.2 and 0.4ng/mg with an intraperitoneal (i.p.) injection, and at 30.9, trace, 4.0 and 0.4ng/mg with oral administration, respectively. There was little difference in hair concentrations between i.p. injection and oral administration. This method was applied to the head hair of two patients who orally took toxic amounts of PB and two volunteers who orally took 100mg of PPH daily for 5d. The hair concentrations of PB in the two patients were 16.2 and 14.7ng/mg, and those of PPH in the two volunteers were 3.3 and 0.1ng/mg.

• 公益社団法人日本薬学会の論文
• 2001-01-01

著者

• Tanaka Einosuke

  University Of Tsukuba

• Nakahara Y

  National Institute Of Health Sciences

• Nakahara Yuji

  National Institute Of Health Sciences

• SAISHO Kazuhiro

  National Institute of Health Sciences

• Saisho K

  National Institute Of Health Sciences

関連論文

• Hair Analysis for Pharmaceutical Drugs.I.Effective Extraction and Determination of Phenobarbital, Phenytoin and Their Major Metabolites in Rat and Human Hair
• Hair analysis for abused and therapeutic drugs
• Hair Analysis for Pharmaceutical Drugs. II. Effective Extraction and Determination of Sildenafil(Viagra^)and Its N-Desmethyl Metabolite in Rat and Human Hair by GC-MS^
• Hair Analysis for Drugs of Abuse. XVIII. 3,4-Methylenedioxymethamphetamine (MDMA) Disposition in Hair Roots and Use in Identification of Acute MDMA Poisoning
• Hair analysis for drugs of abuse XIX. Determination of ephedrine and its homologs in rat hair and human hair
• Abuse of Smoking Methamphetamine Mixed with Tobacco. V.Plasma Metabolites of N-Cyanomethylmethamphetamine, a Pyrolysis Product Formed by Smoking Methamphetamine in Tobacco, and the Species Difference between Mouse and Rat
• Determination of a New Type of Phosphodiesterase-5 Inhibitor, Thioquinapiperifil, in a Dietary Supplement Promoted for Sexual Enhancement
• The disposition into hair of new designer drugs ; methylone, MBDB and methcathinone

スポンサーリンク