Effects of Hydroxylamine on Mitochondrial Monoamine Oxidase in Rat Liver

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The report here is the study as for the effects of NH_2OH on the enzymic reaction of monoamine oxidase (MAO) using rat liver mitochondria. In the short-term reaction (1 min) by MAO, NH_2OH decreased the MAO activity by either tyramine or benzylamine as substrate. In the long-term reaction (60 min), there was an increased activity in the MAO reaction by tyramine and an decreased one in that by benzylamine under various concentrations of NH_2OH. In the long-term reaction, Km-values for tyramine and benzylamine were 1.82×10^<-3.0>M and 2.50×10^<-2.0>M, respectively, which were consistent with those in the presence of NH_2OH. Pursuing the changes of MAO activities by various concentrations of NH_2OH using the tyramine substrate at intervals of 1,10,30,and 60 min, there was a decrease of MAO activity accompanying an increased concentration of NH_2OH after 1 min period, a partial increase after 10 min, and an entire increase in extensive concentrations of NH_2OH after 30 min. In the other substrates such as tryptamine, amylamine, hexylamine, and β-phenylethylamine, the effects of NH_2OH on MAO were similar to those in tyramine. In the butylamine substrate, NH_2OH caused as inhibitory effects as shown in the benzylamine substrate. These results suggest the existence of a complex of MAO enzymes corresponding to monoamine substrates. The multiplicity of MAO enzyme is further discussed in the text.
公益社団法人日本薬学会の論文
1976-04-25