Purification and Identification of Guanosine 3' : 5'-Monophosphate from Higher Plants (Evodiae Fructus)

概要

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A cyclic guanosine monophosphate (GMP)-like substance which can be quantitated both by competitive binding assay and by radioimmunoassay has been found in Evodiae Fructus, an oriental herb. This substance has been extracted and extensively purified by Bio-Rad AG 1×4 and aluminum oxide column chromatographies and thin-layer chromatography (TLC). The purified substance was identified as cyclic GMP, since it showed the same physicochemical properties as authentic cyclic GMP on TLC with various solvents, ultraviolet spectroscopy, and high pressure-liquid chromatography and it was found to be decomposable by cyclic nucleotide specific phosphodiesterase. The existence of cyclic GMP was also demonstrated in several species of Rutaceae. The pharmacological significance of cyclic GMP in this medicinal herb is discussed here.
公益社団法人日本薬学会の論文
1982-07-25