Shear-Rate Dependence of the Intrinsic Viscosity of Sodium Hyaluronate in 0.2 M Sodium Chloride Solution

概要

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The dependence of the intrinsic viscosities ([η]) of seven well-characterized sodium hyaluronate (HA) samples in 0.2 M NaCl solution on shear rates (γ^^・) was investigated using four kinds of viscometers with γ^^・ values ranging from 0.1 to 2000 s^<-1>. Molecular weight distributions of these samples were checked by a gel permeation chromatograph connected to a low-angle laser light scattering photometer. The determination of [η] at zero shear rate ([η]_0) for high molecular weight HA had to be made using low shear viscometers with γ^^・<250s^<-1>, since [η] showed remarkable shear thinning behavior with increasing molecular weight. Double logarithmic plots of [η]_0 vs. M_w (the weight-average molecular weight) for HA in 0.2 M NaCl solution gave a relation expressed by [η]_0=1.99×10^<-4>・M^<0.829>_w for M_w≧40×10^4,where [η]_0 was written in dl/g unit. The shear thinning behavior of [η] of HA in the solvent was analyzed by the Rouse-Zimm bead-spring model proposed by Fixman. Consequently, the γ^^・-dependence of HA in 0.2 M NaCl solution could be well described by this model of large viscosity expansion factors, and suggested that an HA chain in the solvent is a fairly expanded random coil by excluded volume effects.
公益社団法人日本薬学会の論文
1994-08-15