Deamidation at Asparagine-88 in Recombinant Human Interleukin 2

概要

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Reversed-phase high-performance liquid chromatography (RP-HPLC) and protein-chemical analysis revealed that only Asn^<88> in recombinant human interleukin 2 (rIL-2) is liable to be deamidated during a long period of storage in aqueous solutions (pH 5.0) at 25℃, even though there are eight asparagine and six glutamine residues. The deamidation occurred more easily at 40℃ than at 25℃ but did not occur at all at tempratures below 5℃. The biological activity of Asn^<88>-deamidated rIL-2 was found to be almost the same as that of intact rIL-2,whereas its isoelectric point (pI 7.6) is different from that of intact rIL-2 (pI 7.9).
社団法人日本薬学会の論文
1992-04-25