Internalization Model Using Rat Liver Plasma Membrane for Receptor-Mediated Endocytosis of Epidermal Growth Factor

概要

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We have demonstrated the internalization of epidermal growth factor (EGF) using membrane isolated from rat liver. The isolated membrane exhibited a saturation curve of the binding of ^<125>I-EGF. Furthermore, competition between the bindign of ^<125>I-EGF and unlabeled EGF to the isolated membrane was observed. These results were similar to those obtained from whole hepatocytes. In order to confirm whether or not the present experimental system using the isolated membrane can be used for the study of receptor-mediated endocytosis, the internalization of pre-bound EGF in the isolated membrane was assessed by two different methods. First, acid-insensitive ^<125>I-EGF time-dependently increased following incubation at 37℃. Secondly, EGF became inaccessible to the exogenous ^<125>I-anti-EGF antibody when under the same condition. These processes were dependent on adenosine triphosphate, but independent of Ca^<2+> adn stimulated by guanosine triphophate. These results demonstrate that receptor-mediated endocytosis occurred in the isolated membrane.
公益社団法人日本薬学会の論文
1991-12-25