Triton-Induced Proteolysis of Rabbit Intestinal Microvillar Proteins : Inhibition by Ethylenediamine N, N, N', N'-Tetraacetic Acid

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概要

• 論文の詳細を見る

• The addition of Triton X-100 to isolated rabbit intestinal microvilli induced proteolysis of some microvillar proteins. The proteolysis, which was conspicuous in actin, was inhibited by ethylenediamine N, N, N', N'-tetraacetic acid (EDTA) effectively at pH 7.0 but partially at pH 8.6. Phosphoramidon and phenylmethylsulfonyl fluoride showed no inhibitory effect. When microvilli solubilized by Triton X-100 were concentrated, trehalase, a microvillar membrane enzyme, became subject to limited proteolysis, which was also almost completely inhibited by EDTA at pH 7.0. These results show that on solubilization of intestinal microvilli with detergent, care must be taken to avoid induced proteolysis. Most of such proteolysis can be prevented by EDTA at pH 7.0.

• 公益社団法人日本薬学会の論文
• 1985-12-25

著者

• 武居 幸子

  名大・医短

• 小崎 康子

  Research Institute Of Environmental Medicine Nagoya University

• 武居 能樹

  岐阜大・教養

• 横田 邦男

  一宮女短大

• 横田 邦男

  Research Institute of Environmental Medicine Nagoya University

• 武居 幸子

  Biological Institute, Nagoya University

• 武居 能樹

  Research Institute of Environmental Medicine Nagoya University

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• Triton-Induced Proteolysis of Rabbit Intestinal Microvillar Proteins : Inhibition by Ethylenediamine N, N, N', N'-Tetraacetic Acid
• Partial Separation of Isomaltase from Glucoamylase in the Intestinal Brush Border Membrane of the Frog, Rana Japonica
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