Determination of Glucose in Blood using Glucose Oxidase-Peroxidase System and 8-Hydroxyquinoline-p-Anisidine

概要

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An equimolar mixture of 8-Hydroxyquinoline and p-anisidine was found to be oxidized with hydrogen peroxide in the presence of peroxidase giving intense blue color. Application of this mixed reagent to the assay of glucose using glucose oxidase and peroxidase provided a sensitive and reproducible method which did not require deproteinization. Ascorbic acid did not interfere with the color development after pre-incubation of the serum for a short period. Satisfactory correlation between the present method and the conventional phenol-4-aminoantipyrine method was observed.
公益社団法人日本薬学会の論文
1979-02-25