Effects of Temperature and State of Substrate on the Rate of Hydrolysis of Glycerides by Lipase
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Effects of incubation temperature and state of substrate on the substrate specificity of lipases from Pseudomonas fluorescence and Candida cylindracea were studied. The substrate specificity of lipases on single triglyceride emulsions changed with the incubation temperature, and the triglyceride of long chain fatty acids which was poor substrate at 30°became good substrate at 50°or 60°. On the other hand, substrate specificity of lipases on equimolar mixture of single triglycerides was not affected by the incubation temperature or emulsifier, and the pattern of relative rate of hydrolysis was similar to those of single triglycerides at a high temperature. From the results, it was suggested that the substrate specificity observed for single triglycerides may reflected not only the chemical structure of the substrate but also the physical state, and that the mixture of a series of single triglyceride was available for the investigation of substrate specificity of lipase for the chemical structure.
- 公益社団法人日本薬学会の論文
- 1975-03-25
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