Enzymatic Synthesis of 3'-^<32>P-Labeled Coenzyme A from 3'-Dephospho-Coenzyme A.

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概要

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• It has been confirmed that 3'-^<32>P-labeled CoA was enzymatically prepared by incubating 3'-dephospho-CoA with ^<32>P-labeled p-nitrophenyl phosphate and cell suspensions of Proteus mirabilis. The formed labeled CoA with a specific radioactivity of 2 μc per mg. was separated from the reaction mixture by an ECTEOLA cellulose column chromatography. The amount of the labeled CoA was determined enzymatically by phosphotransacetylase-hydroxamic acid assay, spectrophotometrically based on the molecular extinction coefficient of CoA, and radiochemically from the known specific radioactivity of ^<32>P-labeled p-nitrophenyl phosphate.

• 公益社団法人日本薬学会の論文
• 1967-07-25

著者

• 細木 和

  Research Laboratories, Dainippon Pharmaceutical Co., Ltd.,

• 吉村 嘉男

  Research Laboratory, Dainippon Pharmaceutical Co., Ltd.

• 吉村 嘉男

  Research Laboratory Dainippon Pharmaceutical Co. Ltd.

• 黒岡 繁

  Research Laboratory, Dainippon Pharmaceutical Co., Ltd.

• Hosoki K

  Research Laboratories Dainippon Pharmaceutical Co. Ltd.

• 黒岡 繁

  Research Laboratory Dainippon Pharmaceutical Co. Ltd.

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• An Improved Method for Liquid Scintillation Counting of Biological Materials
• Enzymatic Synthesis of 3'-^P-Labeled Coenzyme A from 3'-Dephospho-Coenzyme A.

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