Application of Azotometry. XVII. Determination of Glycosamine.

概要

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A new method for the separatory determination of glycosamine present in polysaccharides was established. Glycosamine generates nitrogen gas by reaction with nitrous acid, but it no longer produces nitrogen gas after reaction with acetylacetone, because the reaction converts it into the corresponding pyrrole derivative. Combination of the two reactions can determine the amount of glycosamine present in the hydrolyzate of polysaccharides. The values of nitrogen gas generated by the reaction of a sample with nitrous acid before and after treatment with acetylacetone are measured by Iwasaki's amino-N Azotometry, and the quantity of glycosamine in the sample is calculated from the difference between the two values. In this method the presence of amino acids is not obstructive.
公益社団法人日本薬学会の論文
1959-02-10