ブタノール-トリウム抽出法による生体液中のオーレオマイシンの比色定量
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概要
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An analytical method, which permits the application of thorium method reported previously by the authors, for determining Aureomycin in biological fluids was proposed. This method is based on the formation of Aureomycin-thorium complex which is soluble in water but insoluble in butanol. Aureomycin was effectively extracted from the sample with butanol when metals were masked with disodium ethylenediaminetetraacetate and salted out with sodium chloride at pH 1〜2. The extracted Aureomycin in butanol was subsequently transferred to an aqueous medium in the form of Aureomycin-thorium complex by a thorium nitrate solution, and its absorbance was measured spectrophotometrically at 400mμ, adjusted to pH 4.0〜4.2 with sodium acetate-acetic acid buffer solution. By this method, as little as 2γ/cc. of Aureomycin Hydrochloride can be successfully determined. The feasibility of employing this method for the determination of Aureomycin in urine and blood was demonstrated. The procedure seems to be adaptable to all forms of pharmaceutical preparations, and fermentation liquors, with minor modification.
- 公益社団法人日本薬学会の論文
- 1955-08-20
著者
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坂口 武一
Pharmaceutical Faculty University Of Chiba
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田口 清水
Pharmaceutical Faculty University Of Chiba
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田口 清水
Pharmaceutical Institute University Of Chiba
関連論文
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- ブタノール-トリウム抽出法による生体液中のオーレオマイシンの比色定量
- テトラサイクリン誘導体の金属鋏合化合物(第2報)トリウムによるオーレオマイシンの比色定量
- テトラサイクリン誘導体の金属鋏合化合物(第3報)硼酸によるオーレオマイシンの比色定量