Polynucleotide-Chitosan Complex, an Insoluble but Reactive Form of Polynucleotide

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概要

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• DNA formed an insoluble complex on mixing with chitosan (poly-D-glucosamine) in solution. The DNA content of the complex was about 50% and the DNA remained insoluble in aqueous media of pH 2-7; e.g., on treatment of the DNA-chitosan complex with phosphate-buffered saline at pH 7 and 37℃ for 26h, the DNA released into the aqueous phase was less than 0.05%. Obviously, DNA and chitosan formed a tight complex due to ionic interactions. The DNA can be solubilized by treatment with 0.1N NaOH. RNA and other polynucleotides formed similar insoluble complexes with chitosan. The DNA attached to chitosan can be digested with a mixture of DNase I and phosphodiesterase. Cytosine residues in the DNA (denatured DNA) can be deaminated by treatment with sodium bisulfite, forming uracil DNA-chitosan. The uracil DNA-chitosan served as a substrate for uracil DNA glycosylase. Using polynucleotide-chitosan as an adsorbent, the affinities of reagents for polynucleotides can be determiend directly. With this technique it was found that carcinogenic heterocyclic amines have an affinity for RNA as well as DNA. The results with homopolyribonucleotide-chitosans as adsorbents for 4 heterocyclic amines indicated that the binding occurs in a purine nucleotide-specific manner. These results suggest that the polynucleotides in the chitosan complex are accessible to enzymes and reagents.This new derivative may be useful in chemical and biological studies of polynucleotides and substances interacting with polynucleotides.

• 公益社団法人日本薬学会の論文
• 1997-08-15

著者

• HAYATSU HIKOYA

  Faculty of Pharmaceutical Sciences, Okayama University

• TANAKA Yuji

  Faculty of Agriculture, Kinki University

• Kubo T

  Faculty Of Pharmaceutical Sciences Okayama University

• Negishi K

  Gene Research Center Okayama University

• Negishi Kazuo

  Gene Research Center

• Hayatsu Hikoya

  Faculty Of Pharmaceutical Sciences Okayama University

• Hayatsu Hikoya

  Faculty Of Pharmaceutical Sciences

• KUBO Takashi

  Faculty of Pharmaceutical Sciences Okayama University

• Kubo Takashi

  Faculty Of Engineering Yokohama National University

• Tanaka Yuji

  Faculty Of Pharmaceutical Sciences Okayama University

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