pH-Dependent Coordination of Metal-Lisinopril Complex Investigated by Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy

スポンサーリンク

概要

• 論文の詳細を見る

• In order to simulate the in vivo binding behavior of angiotensin-converting enzyme(ACE)inhibitors to the zinc-containing active center of ACE, the in vitro interaction between lisinopril and zinc or nickel ions was investigated in aqueous solutions of different pH by using attenuated total reflection(ATR)/Fourier transform infrared(FT-IR)spectroscopy with second-derivative IR spectral analysis. The results indicated that the lisinopril dissociation process occurred in a stepwise fashion during increase in pH. The IR peaks at 1642cm^<-1>(carbonyl stretching of tertiary amide)and at 1582cm^<-1>(asymmetric COO^- stretching)for lisinopril in solution at pH 3.5 shifted to 1606 and 1586cm^<-1> after addition of Ni^<2+> ions, respectively, but there was no marked changes in IR spectra of lisinopril after addition of Zn^<2+> ions. When the Zn^<2+> ions were added to lisinopril solution at pH 5.0, the peak at 1642cm^<-1> also shifted to 1604cm^<-1> and the peak at 1582cm^<-1> shifted to 1586cm^<-1> similar to the changes at PH 3.5 after adding Ni^<2+> ions. However, the peaks at 1582 and 1642cm^<-1> both shifted to 1599cm^<-1> after addition of Ni^<2+> ions at pH 5.0 or at pH 7.3. The peak at 1576cm^<-1> also shifted to 1599cm^<-1> after addition of Zn^<2+> ions to lisinopril solution at pH 7.3. Different coordination sites or types(chelating, bridging or pseudounidentate complex)between lisinopril and Zn^<2+> or Ni^<2+> ions were proposed, based on the separation value between V_<as>(COO^-)and V_s(COO^-), and the shifting of carbonyl groups. Coordination of the secondary amine in lisinopril to metal ions was also evidenced.

• 公益社団法人日本薬学会の論文
• 2002-01-01

著者

• WANG Shun-Li

  Biopharmaceutics Laboratory, Department of Medical Research & Education, Veterans General Hospital-T

• LIN Shan-Yang

  Biopharmaceutics Laboratory, Department of medical Research & Education, Veterans General Hospital-T

• Wang Shun-li

  Biopharmaceutics Laboratory Department Of Medical Research & Education Veterans General Hospital

• Lin Shan-yang

  Biopharmaceutical Laboratory Department Of Medical Reseach And Education Veterans General Hospital-t

• CHUANG Chi-Hasiang

  Biopharmaceutics Laboratory, Department of Medical Research & Education, Veterans General Hospital-T

• Wang Shun-li

  Biopharmaceutics Laboratory Department Of Medical Research & Education Veterans General Hospital

• Lin Shan-yang

  Biopharmaceutics Laboratory Department Of Medical Research & Education Veterans General Hospital

• Chuang Chi-hasiang

  Biopharmaceutics Laboratory Department Of Medical Research & Education Veterans General Hospital

• Chuang Chi-Hsiang

  Biopharmaceutics Laboratory, Department of Medical Research & Education, Veterans General Hospital-Taipei

関連論文

• Transformation of Metastable Forms of Acetaminophen Studied by Thermal Fourier Transform Infrared(FT-IR)Microspectroscopy
• Reaction Kinetics of Solid-state Cyclization of Enalapril Maleate Investigated by Isothermal FT-IR Microscopic System
• Thermal-Dependent Dehydration Process and Intramolecular Cyclization of Lisinopril Dihydrate in the Solid State
• pH-Dependent Coordination of Metal-Lisinopril Complex Investigated by Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy
• Use of Microscopic FT-IR/DSC Combined System for the Study of Glass Transition Temperatures of Polymers
• Compression Behavior of the Different Grades of Ethyl Cellulose

スポンサーリンク